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Distortion was also noticed in samples that contains lipids, especially in the Hoki roe homogenate. Pokhariyal et al. [forty nine] documented the complexity of protein bands within the SDS-PAGE was greater while in the presence of lipid, which triggered distortion of protein bands.
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The protein band distributions in the homogenates of Hoki roe and Gemfish roe have been very similar, with Hoki roe exhibiting more protein fragments over 20 kDa as compared to Gemfish roe, and after the delipidation there was a protein band absence at 60 kDa in the two freeze-dried and fresh new Hoki roe homogenate. Before hydrolysis, the protein bands of your non-delipidation fish roe were all distorted, with new Hoki roe displaying by far the most prominent distortion. In addition, the freeze-dried Hoki roe and Gemfish roe samples in addition to the clean Gemfish slot gacor roe, all exhibited distortion around 3 kDa (Figure 6c).
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